Plasmid

Part:BBa_K4808011:Design

Designed by: Zhao Guichun   Group: iGEM23_AIS-China   (2023-10-10)


pEcCas


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4334
    Illegal EcoRI site found at 8435
    Illegal EcoRI site found at 9944
    Illegal EcoRI site found at 11128
    Illegal EcoRI site found at 12594
    Illegal XbaI site found at 7132
    Illegal SpeI site found at 1124
    Illegal PstI site found at 1805
    Illegal PstI site found at 2760
    Illegal PstI site found at 9439
    Illegal PstI site found at 9686
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4334
    Illegal EcoRI site found at 8435
    Illegal EcoRI site found at 9944
    Illegal EcoRI site found at 11128
    Illegal EcoRI site found at 12594
    Illegal NheI site found at 4093
    Illegal NheI site found at 14303
    Illegal SpeI site found at 1124
    Illegal PstI site found at 1805
    Illegal PstI site found at 2760
    Illegal PstI site found at 9439
    Illegal PstI site found at 9686
    Illegal NotI site found at 1443
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4334
    Illegal EcoRI site found at 8435
    Illegal EcoRI site found at 9944
    Illegal EcoRI site found at 11128
    Illegal EcoRI site found at 12594
    Illegal BglII site found at 13274
    Illegal BamHI site found at 6372
    Illegal BamHI site found at 8374
    Illegal XhoI site found at 2613
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4334
    Illegal EcoRI site found at 8435
    Illegal EcoRI site found at 9944
    Illegal EcoRI site found at 11128
    Illegal EcoRI site found at 12594
    Illegal XbaI site found at 7132
    Illegal SpeI site found at 1124
    Illegal PstI site found at 1805
    Illegal PstI site found at 2760
    Illegal PstI site found at 9439
    Illegal PstI site found at 9686
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4334
    Illegal EcoRI site found at 8435
    Illegal EcoRI site found at 9944
    Illegal EcoRI site found at 11128
    Illegal EcoRI site found at 12594
    Illegal XbaI site found at 7132
    Illegal SpeI site found at 1124
    Illegal PstI site found at 1805
    Illegal PstI site found at 2760
    Illegal PstI site found at 9439
    Illegal PstI site found at 9686
    Illegal AgeI site found at 8209
    Illegal AgeI site found at 9866
    Illegal AgeI site found at 13006
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 8191


Design Notes

none


Source

Li Q, Sun B, Chen J, Zhang Y, Jiang Y, Yang S. A modified pCas/pTargetF system for CRISPR-Cas9-assisted genome editing in Escherichia coli. Acta Biochim Biophys Sin (Shanghai). 2021;53(5):620-627. doi:10.1093/abbs/gmab036

References